Composite

Part:BBa_K2928006:Design

Designed by: Lauren Hartz   Group: iGEM19_ASIJ_Tokyo   (2019-10-15)


Modified 0.19 Wheat Albumin (Ile to Asn) with expression using TDH3, RBS, and ADH3


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

We had to select promoters and terminators of appropriate strength. Additionally, we made the decision to create this construct without a secretion tag, electing instead to lyse the cells prior to analyzing the activity of the synthesized (modified) 0.19 in a system with alpha-amylase.


Source

This part is composed of previously existing parts. The sequence for the original unmodified 0.19 Wheat Albumin is from the one available in the PDB (Protein Database). We subsequently made the modifications explained above.

References